This study presented the optimization of a simple HPLC-UV method for the determination of repaglinide (RPG) in\nhuman plasma. Chromatographic separation followed by UV detection was performed on deproteinized human plasma samples.\nThe separation was carried out on a cosmosil C-18 column (250 mm Ã?â?? 4.6 mm, 5 ?m) with UV detection at 230 nm. The mobile\nphase contained acetonitrile: ammonium acetate buffer pH 4 (80 : 20 v/v). The mobile phase was run isocratically. The flow rate\nof the mobile phase was maintained at 1 ml/min. The linearity of the calibration curve was obtained in the concentration range\nof 10 to 100 ?g/ml for repaglinide and coefficient of correlation (r2) was found to be 0.999. The lowest limit of detection and\nquantification was 10 and 20 ng/ml respectively. No endogenous substances were found to interfere with the peaks of drug and\nplasma. The intra-day and inter-day coefficient of variations was less for all the selected concentrations. This method was time\nefficient and samples are easy to prepare with minimum dilution. So, it can be applied for monitoring repaglinide in human\nplasma.
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